A high-speed low-latency interconnect interface (HLII) for silicon interposer interconnection is provided. The HLII is configured to perform large-scale input/output (I/O) interconnection on a silicon interposer, and includes a physical link (PL) and an LL (LL). The LL receives a data signal, a configuration signal, and a control signal of logical resource inside a chiplet, and can complete data conversion, parity check, training, channel repair, instruction stream generation, and other functions for the PL. The PL receives and transmits a data signal converted by the LL. The PL includes a high-speed I/O port, a first input first output (FIFO), and related control logic. The high-speed I/O port of the PL is compatible with both a double date rate (DDR) transmission mode and a single data rate (SDR) transmission mode.