The present application discloses a multi-node safe locking device. A guiding rail is disposed on a chassis, to enable a locking rod to translate perpendicularly to the moving direction of node modules. A guiding plate is assembled on the node modules, guiding grooves are disposed on the guiding plate, and the guiding grooves match with a plurality of limiting columns that protrude out of the locking rod. When the node modules moves, the limiting columns move relatively to the guiding grooves in a guiding path, to cause the locking rod to translate. When one of the node modules is pulled out, the guiding groove causes the locking rod to translate to the locking position, to block the other node module from being pulled out, the limiting column is separated from the notch of the guiding groove, and the guiding plate moves out along with the node module.