A pixel arrangement structure and its driving method, as well as a display substrate and its manufacturing method are provided. The pixel arrangement structure comprises: pixel units arranged in an array, where each of the pixel units comprises a first sub-pixel (11) and a second sub-pixel (12), the first sub-pixel (11) includes a first quantum dot light emitting diode, the second sub-pixel (12) includes an inorganic light emitting diode, a light emitting color of the second sub-pixel is identical to that of the first sub-pixel, or the light emitting color of the second sub-pixel is white. The display substrate has a long service life and wide color gamut.