Embodiments for video encoding and video decoding are described. One example method includes performing a conversion between a video and a bitstream of the video according to a rule, wherein the rule specifies that only one of two conditions is true when a number of subpictures minus 1 indicated by a first syntax element in a sequence parameter set is greater than zero and when a current subpicture with a subpicture index subpicIdx of a first video picture of the video has a second syntax element in the sequence parameter set indicates that a subpicture with the subpicture index subpicIdx of each video picture of the video in a coded layer video sequence is treated as a video picture in a decoding process that excludes an in-loop filtering operation.