This present invention discloses a detachable probe cover for an ear thermometer and a manufacturing method thereof. The detachable probe cover for the ear thermometer is for being mounted onto a measuring probe of the ear thermometer, wherein a combining mechanism is provided at a bottom of the measuring probe and the detachable probe cover comprises a main body of a hollow structure and a base, in which the main body has an open end and a closed end opposite to the open end, and the hollow structure has a diameter gradually reducing from the open end toward the closed end.