A method for manufacturing a display substrate includes forming a plastic base substrate. A blocking layer is formed on a surface of the plastic base substrate by depositing a first material and a second material that are distinct. A substrate includes a plastic base substrate and a blocking layer formed at surfaces of the plastic base substrate and having a first layer and a second layer alternatingly. The first layer and second layer include continuously changing component ratio of a first material to a second material. The blocking layer effectively blocks moisture and/or oxygen.