Invention Application
- Patent Title: APPLICATION OF VIABLE CELL MONITOR TO CRYOPRESERVATION OF PLANT CELLS
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Application No.: US16347429Application Date: 2017-11-22
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Publication No.: US20190274299A1Publication Date: 2019-09-12
- Inventor: Meijin Guo , Jiarui Li , Zejian Wang , Yuxia Chen , Yingping Zhuang
- Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
- Priority: CN201611041562.6 20161122
- International Application: PCT/CN2017/112299 WO 20171122
- Main IPC: A01N1/02
- IPC: A01N1/02 ; G01N27/22

Abstract:
The present disclosure relates to the application of a viable cell monitor to the cryopreservation of plant cells. The viable cell monitor is, for the first time, used in a method for rapidly evaluating the effect of cell freezing-preservation. The effect of a cryoprotectant on cell preservation is evaluated through the change in the capacitance of viable cells before and after cell freezing and resuscitation. Viable cell electrodes can provide a quick and accurate characterization of viable cell concentrations. Combining the viable cell capacitance with the detection result of 2,3,4-triphenyl tetrazolium chloride (TTC), a rapid and quantitative evaluation of the effect of a freezing method on cell preservation can be achieved, thus overcoming the low effectiveness and non-quantification of previous cell preservation methods. Compared with traditional methods, the method of the present disclosure can improve the comprehensive score of cell cryopreservation by 5 times or more.
Public/Granted literature
- US11291200B2 Application of viable cell monitor to cryopreservation of plant cells Public/Granted day:2022-04-05
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