Invention Application
- Patent Title: METHODS OF PRODUCING AGGREGATE-FREE MONOMERIC DIPHTHERIA TOXIN FUSION PROTEINS AND THERAPEUTIC USES
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Application No.: US17549845Application Date: 2021-12-13
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Publication No.: US20220204579A1Publication Date: 2022-06-30
- Inventor: William R. Bishai , John R. Murphy , Laurene Cheung , Shashank Gupta , Cynthia K. Bullen
- Applicant: THE JOHNS HOPKINS UNIVERSITY , TRUSTEES OF BOSTON UNIVERSITY
- Applicant Address: US MD Baltmore; US MA Boston
- Assignee: THE JOHNS HOPKINS UNIVERSITY,TRUSTEES OF BOSTON UNIVERSITY
- Current Assignee: THE JOHNS HOPKINS UNIVERSITY,TRUSTEES OF BOSTON UNIVERSITY
- Current Assignee Address: US MD Baltmore; US MA Boston
- Main IPC: C07K14/55
- IPC: C07K14/55 ; C07K14/34 ; C12N15/77
Abstract:
The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.
Public/Granted literature
- US11965009B2 Methods of producing aggregate-free monomeric diphtheria toxin fusion proteins and therapeutic uses Public/Granted day:2024-04-23
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