Proposed are a method and device for regularizing rapid three-dimensional tomographic imaging using a machine-learning algorithm. A method for regularizing three-dimensional tomographic imaging using a machine-learning algorithm according to an embodiment comprises the steps of: measuring a three-dimensional tomogram of a cell to acquire a raw tomogram of the cell; acquiring a regularized tomogram by using a regularization algorithm; and learning the relationship between the raw tomogram and the regularized tomogram through machine-learning.