An insulation inspection device includes: a conveyance unit that conveys a laminated electrode in which a first separator, a first electrode plate, a second separator, and a second electrode plate are laminated; a pressure roll that presses the laminated electrode against the conveyance unit; a first terminal electrically connected to the first electrode plate; a second terminal electrically connected to the second electrode plate, further electrically connected to the conveyance unit when the first separator is disposed on the conveyance unit side, and further electrically connected to the pressure roll when the first separator is disposed on the pressure roll side; and an insulation inspection unit that applies a voltage to the laminated electrode to inspect insulation condition of the laminated electrode.