An electronic apparatus and a hotkey prompt method thereof are provided. The method includes the following steps. A power-on self-test (POST) procedure of a basic input output system (BIOS) is performed. During the POST procedure, a display panel of a keyboard module is controlled to display a first keyboard layout, so as to display at least one hotkey corresponding to at least one hotkey function of the BIOS via the display panel. After a hotkey input operation is received via the keyboard, a first hotkey function of the at least one hotkey function of the BIOS is performed. The hotkey input operation is used to enable the first hotkey function. In response to performing the first hotkey function, the display panel of the keyboard module is controlled to display a second keyboard layout.