A β-glycosidase SS-BGL mutant for modifying ginsenoside and an application thereof are provided, which relate to the field of genetic engineering technologies. The β-glycosidase SS-BGL mutant is a mutant mutating asparagines at 128th position and 302th position of the amino acid sequence as shown in SEQ ID NO: 1 of β-glycosidase SS-BGL into aspartic acids respectively. The β-glucosidase SS-BGL mutant improves the thermal stability of the natural β-glucosidase SS-BGL at extremely high temperature, and is more conducive to the application of SS-BGL in ginsenoside preparation.