A dissolved oxygen measurement system, and a method for calibrating a dissolved oxygen analyzer are provided. The method includes directing a pressurized cleaning fluid, having a known oxygen content, upon a sensing surface of a dissolved oxygen sensor disposed in a fluid. Then, the analyzer waits until the dissolved oxygen sensor response to the pressurized cleaning fluid is relatively stable, and stores a calibration value relative to the dissolved oxygen sensor response to the pressurized cleaning fluid. A dissolved oxygen measurement system is also provided. The system includes a dissolved oxygen sensor disposed in a fluid and a first source of a first compressed cleaning fluid that has a first dissolved oxygen content. A dissolved oxygen analyzer is coupled to the dissolved oxygen sensor and to the first valve and is configured to obtain a first calibration value related to the first dissolved oxygen content.