Invention Grant
US07642064B2 Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
有权
酶催化的金属沉积用于增强感兴趣的分析物的检测
- Patent Title: Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
- Patent Title (中): 酶催化的金属沉积用于增强感兴趣的分析物的检测
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Application No.: US11015646Application Date: 2004-12-20
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Publication No.: US07642064B2Publication Date: 2010-01-05
- Inventor: Christopher Bieniarz , Michael Farrell
- Applicant: Christopher Bieniarz , Michael Farrell
- Applicant Address: US AZ Tucson
- Assignee: Ventana Medical Systems, Inc.
- Current Assignee: Ventana Medical Systems, Inc.
- Current Assignee Address: US AZ Tucson
- Agency: Sterne, Kessler, Goldstein & Fox P.L.L.C.
- Main IPC: C12Q1/42
- IPC: C12Q1/42 ; C12Q1/44 ; C12Q1/54 ; G01N33/53 ; G01N33/543 ; G01N33/544 ; G01N33/551
Abstract:
The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition.
Public/Granted literature
- US20050100976A1 Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest Public/Granted day:2005-05-12
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