Disclosed is a vehicle body structure of a motorcycle which intends to make maintenance of a rear suspension easy and arranges the rear suspension in a compact manner. The vehicle body structure includes a vehicle body frame, a swing arm, and a rear suspension interposed between the vehicle body frame and the swing arm. The vehicle body frame includes a front frame body having right and left main frame members, and a rear frame body having right and left rear frame members extending to a rear side from rear end portions of the main frame members. The rear suspension is arranged so as to be eccentric to a right direction of the vehicle body from a center of the vehicle width. The rear frame body has a layout space for the rear suspension in a lower side of the right rear frame member.