The invention concerns a device for locking a dental instrument having a rotating spindle, formed by a hollow spindle and adapted to receive the head of an instrument, and a locking spring formed by a lower ring extending on a common side by two branches or a plurality of elastic branches. Each branch is provided with a pin engaged in an annular groove provided in the head of the instrument. There is a push-button having, in its underside, a central conical pressure head adapted to space apart radially the branches of the locking spring to release the pins from the annular groove of the instrument. The locking branches are housed in the slots provided in the rotary spindle to lock in rotation the spring relative to the rotary spindle, the spring being moreover maintained axially between the push-button and a shoulder of the rotary spindle.