Invention Grant
- Patent Title: Methods of producing and sequencing modified polynucleotides
- Patent Title (中): 生产和测序修饰的多核苷酸的方法
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Application No.: US11476423Application Date: 2006-06-28
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Publication No.: US07645866B2Publication Date: 2010-01-12
- Inventor: Douglas R. Smith , Kevin J. McKernan
- Applicant: Douglas R. Smith , Kevin J. McKernan
- Applicant Address: US CA Carlsbad
- Assignee: Life Technologies Corporation
- Current Assignee: Life Technologies Corporation
- Current Assignee Address: US CA Carlsbad
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12Q1/68 ; C12P19/34 ; B01L3/00
Abstract:
The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
Public/Granted literature
- US20070026438A1 Methods of producing and sequencing modified polynucleotides Public/Granted day:2007-02-01
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