A blood vessel locating and stabilizing device including a transparent planar device that may be used to locate and stabilize a blood vessel so that the blood vessel is less likely to roll when it is punctured with a needle. The device is planar and includes a transparent region and a recess formed in a distal side surface that is configured to locate and stabilize a blood vessel. In a method of using the present device, the planar member is gripped along a first and second major surfaces of the device, the recess is positioned over the targeted blood vessel, and a force is applied to the device so that the blood vessel is located and stabilized.