Invention Grant
US07659066B2 Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
失效
通过分子生物学方法检测和区分感染法氏囊病病毒（IBDV）菌株
- Patent Title: Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
- Patent Title (中): 通过分子生物学方法检测和区分感染法氏囊病病毒（IBDV）菌株
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Application No.: US11268341Application Date: 2005-11-07
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Publication No.: US07659066B2Publication Date: 2010-02-09
- Inventor: Abdul Rahman Omar , Mohd Hair-Bejo , Ideris Aini , Hairul Aini Hamzah
- Applicant: Abdul Rahman Omar , Mohd Hair-Bejo , Ideris Aini , Hairul Aini Hamzah
- Applicant Address: MY Ehsan
- Assignee: Universiti Putra Malaysa
- Current Assignee: Universiti Putra Malaysa
- Current Assignee Address: MY Ehsan
- Agency: Wolf, Greenfield & Sacks, P.C.
- Priority: MYPI20044610 20041105
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H21/04
Abstract:
The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values.
Public/Granted literature
- US20060099574A1 Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method Public/Granted day:2006-05-11
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