Invention Grant
- Patent Title: Reaction system for performing in the amplification of nucleic acids
- Patent Title (中): 用于在核酸扩增中进行的反应体系
-
Application No.: US11830283Application Date: 2007-07-30
-
Publication No.: US07659096B2Publication Date: 2010-02-09
- Inventor: Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
- Applicant: Martin Alan Lee , Hilary Bird , Dario Lyall Leslie , David James Squirrell , John Shaw , David Wenn , Julie Deacon
- Applicant Address: GB Salisbury, Wiltshire
- Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland
- Current Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland
- Current Assignee Address: GB Salisbury, Wiltshire
- Agency: Kilpatrick Stockton LLP
- Priority: GB9922971.8 19990929; GB0003743 20000929
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34

Abstract:
A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
Public/Granted literature
- US20080176232A1 Reaction System for Performing in the Amplification of Nucleic Acids Public/Granted day:2008-07-24
Information query