Invention Grant
US07659103B2 Nucleic acid construct containing fulllength genome of human hepatitis C virus, recombinant fulllength virus genome-replicating cells having the nucleic acid construct transferred thereinto and method of producing hepatitis C virus Particle
有权
含有人丙型肝炎病毒的全长基因组的核酸构建体，转移有核酸构建体的重组全长病毒基因组复制细胞及其生产丙型肝炎病毒的方法
- Patent Title: Nucleic acid construct containing fulllength genome of human hepatitis C virus, recombinant fulllength virus genome-replicating cells having the nucleic acid construct transferred thereinto and method of producing hepatitis C virus Particle
- Patent Title (中): 含有人丙型肝炎病毒的全长基因组的核酸构建体，转移有核酸构建体的重组全长病毒基因组复制细胞及其生产丙型肝炎病毒的方法
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Application No.: US10589902Application Date: 2005-02-21
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Publication No.: US07659103B2Publication Date: 2010-02-09
- Inventor: Takaji Wakita , Takanobu Kato , Tomoko Date , Michiko Miyamoto , Jun-ichi Tanabe , Saburo Sone
- Applicant: Takaji Wakita , Takanobu Kato , Tomoko Date , Michiko Miyamoto , Jun-ichi Tanabe , Saburo Sone
- Applicant Address: JP Tokyo JP Tokyo
- Assignee: Tokyo Metropolitan Organization For Medical Research,Toray Industries, Inc.
- Current Assignee: Tokyo Metropolitan Organization For Medical Research,Toray Industries, Inc.
- Current Assignee Address: JP Tokyo JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2004-045489 20040220
- International Application: PCT/JP2005/003232 WO 20050221
- International Announcement: WO2005/080575 WO 20050901
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C12N7/00 ; C12N7/01 ; C12N7/02 ; A61K39/29 ; C07H21/00 ; A01N63/00
Abstract:
The present invention provides a method for replicating efficiently an RNA containing fulllength HCV genomic sequence and a method for producing HCV virus particles containing fulllength HCV replicon RNA or fulllength HCV genomic RNA by using a cell culture system. Further, the present invention relates to a method for producing hepatitis C virus particles which comprises culturing a cell, into which a replicon RNA comprising a nucleotide sequence comprising a fulllength genomic RNA sequence of hepatitis C virus of the genotype 2a, at least one selectable marker gene and/or at least one reporter gene and at least one IRES sequence or the fulllength genomic RNA of hepatitis C virus of the genotype 2a is introduced, and generating virus particles in the culture medium. Still further the present invention relates also to a hepatitis C vaccine and an antibody against hepatitis C virus particles.
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