Invention Grant
- Patent Title: Process for producing physiologically active protein using genetically modified silkworm
- Patent Title (中): 使用转基因蚕生产生理活性蛋白的方法
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Application No.: US10506327Application Date: 2003-03-06
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Publication No.: US07659112B2Publication Date: 2010-02-09
- Inventor: Shingo Hiramatsu , Takashi Tanaka , Katsushige Yamada , Toshiki Tamura
- Applicant: Shingo Hiramatsu , Takashi Tanaka , Katsushige Yamada , Toshiki Tamura
- Applicant Address: JP Tokyo
- Assignee: Toray Industries, Inc.
- Current Assignee: Toray Industries, Inc.
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2002-060374 20020306; JP2002-268726 20020913
- International Application: PCT/JP03/02675 WO 20030306
- International Announcement: WO03/074699 WO 20030912
- Main IPC: C12N15/00
- IPC: C12N15/00 ; C12P21/00

Abstract:
The present invention provides a genetic engineering material for insects that enables a target protein to be purified easily, without requiring the use of recombinant baculovirus, while simultaneously providing a process for producing exogenous protein using that genetic engineering material. A gene recombinant silkworm is obtained by inserting an exogenous protein gene such as a cytokine gene coupled to a promoter that functions in silk glands into a silkworm chromosome. An exogenous protein such as a cytokine is then extracted and purified from the silk glands or cocoon of that silkworm or its offspring. A large amount of exogenous protein can be produced within silk gland cells, outside silk gland cells or in silk thread or a cocoon by inserting an expression gene cassette, in which the DNA sequence of the 3′ terminal portion and the DNA sequence of the 5′ terminal portion of fibroin H chain gene are fused to the exogenous protein gene, into silk gland cells and so forth.
Public/Granted literature
- US20050177877A1 Process for producing physiologically active protein using genetically modified silkworm Public/Granted day:2005-08-11
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