Invention Grant
- Patent Title: Method for the in vitro synthesis of short double stranded RNAs
- Patent Title (中): 短双链RNA的体外合成方法
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Application No.: US10494663Application Date: 2002-10-30
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Publication No.: US07659391B2Publication Date: 2010-02-09
- Inventor: Marianne Denise De Backer , Adam N. Harris
- Applicant: Marianne Denise De Backer , Adam N. Harris
- Applicant Address: BE Beerse
- Assignee: Janssen Pharmaceutica N.V.
- Current Assignee: Janssen Pharmaceutica N.V.
- Current Assignee Address: BE Beerse
- International Application: PCT/EP02/12165 WO 20021030
- International Announcement: WO03/040294 WO 20030515
- Main IPC: C07H21/00
- IPC: C07H21/00
Abstract:
The present invention relates to the field of synthesis of short double-stranded RNAs. An in vitro transcription method using bacteriophage polymerases and target sequence-specific single-stranded DNA oligonucleotides as templates is disclosed. The present invention finds particularly advantageous use in the synthesis of short interfering RNAs (siRNAs) that have been shown to function as key intermediates in triggering sequence-specific RNA degradation during posttranscriptional gene silencing in plants and RNA interference in invertebrates and vertebrate systems.
Public/Granted literature
- US20040259097A1 Method for the in vitro synthesis of short double stranded rnas Public/Granted day:2004-12-23
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