Invention Grant
US07659443B2 Method of constructing nucleus-implanted egg, parthenogenetic embryo and parthenogenetic mammal
有权
构建核移植卵,孤雌胚胎和孤雌生殖哺乳动物的方法
- Patent Title: Method of constructing nucleus-implanted egg, parthenogenetic embryo and parthenogenetic mammal
- Patent Title (中): 构建核移植卵,孤雌胚胎和孤雌生殖哺乳动物的方法
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Application No.: US12213385Application Date: 2008-06-18
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Publication No.: US07659443B2Publication Date: 2010-02-09
- Inventor: Tomohiro Kono , Yayoi Obata , Manabu Kawahara
- Applicant: Tomohiro Kono , Yayoi Obata , Manabu Kawahara
- Applicant Address: JP Tokyo
- Assignee: Tokyo University of Agriculture Educational Corporation
- Current Assignee: Tokyo University of Agriculture Educational Corporation
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2003-286543 20030805; JP2007-171707 20070629
- Main IPC: C12N15/00
- IPC: C12N15/00 ; A01K67/027

Abstract:
Disclosed is a method for constructing a nucleus-implanted egg, a parthenogenetic embryo and for producing a parthenogenetic mammal each having 2 haploid genome sets originating in mammalian ova, and provides methods of constructing a nucleus-implanted egg having a haploid genome set derived from primitive ovarian follicle egg (ng ovum) and a haploid genome set from MII phase (second meiosis metaphase) egg (fg ovum), a parthenogenetic embryo and a parthenogenetic mammal, including steps (1) introducing ng ovum into a nucleus-deleted deleted germinal vesicle stage (GV) egg, developing the obtained egg to MII phase by in vitro maturing and culturing to prepare a first nucleus-implanted egg, and (2) extracting MII phase chromosome from the first nucleus-implanted egg and introducing it into other fg ovum to prepare a second nucleus-implanted egg, wherein a ng or fg ovum from which an imprinted gene undergoing epigenetic modification during sperm generation is used.
Public/Granted literature
- US20090031437A1 Method of constructing nucleus-implanted egg, parthenogenetic embryo and parthenogenetic mammal Public/Granted day:2009-01-29
Information query
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