A device (10) for clamping and separating a length of tubing. The device (10) comprises first and second subassemblies (12,14) for, in their closed positions, clamping the tubing at first and second locations, respectively, and at least weakening the tubing for subsequent separation. The subassemblies (12,14) face each other and are connected by a breakable connection. Each subassembly (12,14) includes clamping elements (26,34) for clamping the tubing, a perforating or cutting element (28) for perforating or otherwise weakening or cutting the tubing, and locking elements (24,32) for locking the subassembly (12,14) in the closed position. The perforating element (28) may be at least one hard plastic or metal spike. Once each subassembly (12,14) is locked in the closed position, the breakable connection is broken to result in respective lengths of clamped tubing.