Invention Grant
- Patent Title: Method for silver staining of biological substances
- Patent Title (中): 生物物质银染方法
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Application No.: US11663632Application Date: 2005-09-27
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Publication No.: US07662640B2Publication Date: 2010-02-16
- Inventor: Asako Okawa
- Applicant: Asako Okawa
- Applicant Address: JP Kyoto
- Assignee: Kyoto University
- Current Assignee: Kyoto University
- Current Assignee Address: JP Kyoto
- Agency: Greenblum & Bernstein, P.L.C.
- Priority: JP2004-282227 20040928
- International Application: PCT/JP2005/017769 WO 20050927
- International Announcement: WO2006/035781 WO 20060406
- Main IPC: G01N1/30
- IPC: G01N1/30

Abstract:
A method for silver staining a gel on which biological substances such as nucleic acids and proteins are separated by electrophoresis, which comprises at least the following steps: (a) the step of immersing an untreated gel after the electrophoresis in an aqueous solution of sodium thiosulfate; (b) the step of immersing the gel obtained in the step (a) in a mixture of ethanol and an aqueous solution of sodium acetate; and (c) the step of immersing the gel obtained in the step (b) in a mixture of ethanol and an aqueous solution of silver nitrate.
Public/Granted literature
- US20070292961A1 Method for Silver Staining of Biological Substances Public/Granted day:2007-12-20
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