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US07662640B2 Method for silver staining of biological substances 失效
生物物质银染方法

  • Patent Title: Method for silver staining of biological substances
  • Patent Title (中): 生物物质银染方法
  • Application No.: US11663632
    Application Date: 2005-09-27
  • Publication No.: US07662640B2
    Publication Date: 2010-02-16
  • Inventor: Asako Okawa
  • Applicant: Asako Okawa
  • Applicant Address: JP Kyoto
  • Assignee: Kyoto University
  • Current Assignee: Kyoto University
  • Current Assignee Address: JP Kyoto
  • Agency: Greenblum & Bernstein, P.L.C.
  • Priority: JP2004-282227 20040928
  • International Application: PCT/JP2005/017769 WO 20050927
  • International Announcement: WO2006/035781 WO 20060406
  • Main IPC: G01N1/30
  • IPC: G01N1/30
Method for silver staining of biological substances
Abstract:
A method for silver staining a gel on which biological substances such as nucleic acids and proteins are separated by electrophoresis, which comprises at least the following steps: (a) the step of immersing an untreated gel after the electrophoresis in an aqueous solution of sodium thiosulfate; (b) the step of immersing the gel obtained in the step (a) in a mixture of ethanol and an aqueous solution of sodium acetate; and (c) the step of immersing the gel obtained in the step (b) in a mixture of ethanol and an aqueous solution of silver nitrate.
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