Invention Grant
- Patent Title: Method for producing recombinant trypsin
- Patent Title (中): 重组胰蛋白酶的制备方法
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Application No.: US11853483Application Date: 2007-09-11
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Publication No.: US07666629B2Publication Date: 2010-02-23
- Inventor: Rainer Muller , Stephan Glaser , Frank Geipel , Johann-Peter Thalhofer , Bernhard Rexer , Claus Schneider , Michael Ratka , Stephanie Ronning , Hellmut Eckstein , Claudia Giessel
- Applicant: Rainer Muller , Stephan Glaser , Frank Geipel , Johann-Peter Thalhofer , Bernhard Rexer , Claus Schneider , Michael Ratka , Stephanie Ronning , Hellmut Eckstein , Claudia Giessel
- Applicant Address: US IN Indianapolis
- Assignee: Roche Diagnostics Operations, Inc.
- Current Assignee: Roche Diagnostics Operations, Inc.
- Current Assignee Address: US IN Indianapolis
- Agency: Barnes & Thornburg LLP
- Priority: EP01102342 20010201
- Main IPC: C12N15/62
- IPC: C12N15/62 ; C12N9/76 ; C12N15/79 ; C12N15/81
Abstract:
The present invention concerns a method for producing recombinant trypsin from porcine pancreas in Pichia pastoris which is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to β-trypsin and autolysis of β-trypsin by α-trypsin into ε-trypsin and from there into inactive peptides.
Public/Granted literature
- US20080064084A1 Method For Producing Recombinant Trypsin Public/Granted day:2008-03-13
Information query
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