A positioning structure for the male reproductive organ, and more particularly provides a positioning structure which contributes to the hygiene, health and comfort of the male reproductive organ, and mainly includes a main body, a spacing portion, a holding portion and a positioning portion, wherein the main body further comprises at least one lower retaining portion and a waistband portion. The present invention is able to avoid the pants crotch, and enables upwardly centrally fixing the position of the penis to prevent it from falling down to one side, thereby resolving the problems of ill health, unhygienic state and discomfort of the penis resulting from constant skin contact between the penis and the scrota, while at the same time effectively preventing the problem of compression deformation of the penis when an erection occurs when wearing tight-fitting pants.