A specimen removal apparatus includes a pouch assembly fabricated from a flexible membrane, a pouch support, a drawstring having a knot and forming a noose disposed circumferentially around a mouth of the pouch assembly, an endoscopic tubular portion, and a drive rod. The pouch assembly includes a plurality of circumferentially disposed guide members advantageously circumferentially spaced apart to define gaps therebetween. The guide members are disposed in a circumferential pathway proximal to the mouth of the pouch assembly. When the drawstring is pulled, the knot is stopped at an end of a guide member and the noose is closed, thereby closing the mouth of the pouch assembly. The pouch assembly is detachable from the apparatus.