Invention Grant
- Patent Title: Use of mutagenic DNA polymerase for producing random mutations
- Patent Title (中): 使用诱变型DNA聚合酶产生随机突变
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Application No.: US10416424Application Date: 2001-11-07
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Publication No.: US07670809B2Publication Date: 2010-03-02
- Inventor: Christophe Cazaux , Jean Sébastien Hoffmann , Thierry Louat , Laurence Servant , Khalil Bouayadi , Hakim Kharrat
- Applicant: Christophe Cazaux , Jean Sébastien Hoffmann , Thierry Louat , Laurence Servant , Khalil Bouayadi , Hakim Kharrat
- Applicant Address: FR Labege
- Assignee: Millegen
- Current Assignee: Millegen
- Current Assignee Address: FR Labege
- Agency: Young & Thompson
- Priority: FR0014333 20001108
- International Application: PCT/FR01/03450 WO 20011107
- International Announcement: WO02/38756 WO 20020516
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
The invention concerns a method for random mutagenesis comprising the replication of a DNA sequence in the presence of an efficient amount of at least a mutase, for example a Pol β, the random mutagenesis rate being at least of the order of 1 mutation for 400 base pairs. The replication product, optionally recombined and amplified, is cloned in an expression vector so as to generate mutated polypeptides which will be selected on the basis of the desired property or properties.
Public/Granted literature
- US20040110294A1 Use of mutagenic dna polymerase for producing random mutations Public/Granted day:2004-06-10
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