Invention Grant
US07670824B2 Multicopy-integration of heterologous genes and expression in Methylobacterium
有权
异源基因的多重整合和在甲基杆菌中的表达
- Patent Title: Multicopy-integration of heterologous genes and expression in Methylobacterium
- Patent Title (中): 异源基因的多重整合和在甲基杆菌中的表达
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Application No.: US11492822Application Date: 2006-07-26
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Publication No.: US07670824B2Publication Date: 2010-03-02
- Inventor: Carlos B. Miguez , Young-Jun Choi
- Applicant: Carlos B. Miguez , Young-Jun Choi
- Applicant Address: CA Ottawa
- Assignee: National Research Council of Canada
- Current Assignee: National Research Council of Canada
- Current Assignee Address: CA Ottawa
- Agent Johanna Coutts
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12N15/00 ; C12N15/74
Abstract:
The integration of genes into methylobacterium, such as M. extorquens ATCC is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (PmxaF). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for M. extorquens.
Public/Granted literature
- US20080026005A1 Multicopy-integration of heterologous genes and expression in methylobacterium Public/Granted day:2008-01-31
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