Methods for fabricating semiconductor structures with backside stress layers are provided. In one exemplary embodiment, the method comprises the steps of providing a semiconductor device formed on and within a front surface of a semiconductor substrate. The semiconductor device comprises a channel region. A plurality of dielectric layers is formed overlying the semiconductor device. The plurality of dielectric layers comprises conductive connections that are in electrical communication with the semiconductor device. A backside stress layer is formed on a back surface of the semiconductor substrate. The backside stress layer is configured to apply to the channel region of the semiconductor device a uniaxial compressive or tensile stress that, with stresses applied by the plurality of dielectric layers, results in an overall stress exerted on the channel region to achieve a predetermined overall strain of the channel region.