A multi-channel communication circuit includes a master device, a plurality of slave devices, and a multiplexer (MUX). A transmitting pin and a receiving pin of a serial interface of the master device are respectively connected to a first data input pin and a first data output pin of the MUX. Two control pins of the serial interface of the master device are connected to two selecting pins of the MUX. Four pins of the serial interface of the master device are connected to a power pin of the MUX. Transmitting pins of a serial interface of each slave device are respectively connected to a plurality of second data input pins of the MUX and receiving pins of a serial interface of each slave device are respectively connected to a plurality of second data output pins of the MUX, the master device communicates with one slave device via transmitting a corresponding selecting signal to the two selecting pins of the MUX to select one slave device.