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US07674582B2 Methods for amplification monitoring and gene expression profiling, involving real time amplification reaction sampling 有权
扩增监测方法和基因表达谱，涉及实时扩增反应抽样

  • Patent Title: Methods for amplification monitoring and gene expression profiling, involving real time amplification reaction sampling
  • Patent Title (中): 扩增监测方法和基因表达谱，涉及实时扩增反应抽样
  • Application No.: US11452455
    Application Date: 2006-06-13
  • Publication No.: US07674582B2
    Publication Date: 2010-03-09
  • Inventor: Vladimir I. Slepnev
  • Applicant: Vladimir I. Slepnev
  • Applicant Address: US MA Mansfield
  • Assignee: Primeradx, Inc.
  • Current Assignee: Primeradx, Inc.
  • Current Assignee Address: US MA Mansfield
  • Agent Mark J. FitzGerald
  • Main IPC: C12Q1/68
  • IPC: C12Q1/68 ;  C12P19/34
Methods for amplification monitoring and gene expression profiling, involving real time amplification reaction sampling
Abstract:
The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.
Abstract(Chinese):

本发明涉及监测一个或多个感兴趣的核酸序列的扩增的方法。 更具体地，本发明涉及实时监测感兴趣序列的扩增的方法。 本文公开的方法提供用于监测来自单个样品的一个序列或两个或更多个序列的扩增的方法，以及用于监测来自两个或更多个样品的一个或多于一个序列的扩增的方法。 本发明的监测方法允许改进一种或多种原始样品中一种或多种靶核酸，特别是靶RNA种类的丰度的确定。

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