Invention Grant
- Patent Title: Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
- Patent Title (中): 使用非侵入性切割反应检测核酸的方法和组合物
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Application No.: US11639781Application Date: 2006-12-15
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Publication No.: US07678541B2Publication Date: 2010-03-16
- Inventor: Joseph A. Sorge , Scott Happe , Andrew Firmin
- Applicant: Joseph A. Sorge , Scott Happe , Andrew Firmin
- Applicant Address: US MA Marlborough
- Assignee: HoLogic, Inc.
- Current Assignee: HoLogic, Inc.
- Current Assignee Address: US MA Marlborough
- Agent Theodore Allen; Paul Nuzzi
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H21/04
Abstract:
The invention provides methods, compositions and kits for generating a signal indicative of the presence of a target nucleic acid sequence in a sample comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid sequence with upstream and downstream oligonucleotides, and cleaving the cleavage structure with a nuclease to generate a signal. The presence of a detectable signal is indicative of the presence of a target nucleic acid sequence and a non-invasive cleavage structure.
Public/Granted literature
- US20080299549A1 Methods and compositions for the detection of a nucleic acid by a cleavage reaction Public/Granted day:2008-12-04
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