Invention Grant
- Patent Title: Method for amplifying genomic DNA
- Patent Title (中): 扩增基因组DNA的方法
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Application No.: US12214044Application Date: 2008-06-16
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Publication No.: US07678543B2Publication Date: 2010-03-16
- Inventor: Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
- Applicant: Takashi Imai , Mayumi Iwakawa , Yuichi Michikawa
- Applicant Address: JP Anagawa
- Assignee: National Institute of Radiological Sciences
- Current Assignee: National Institute of Radiological Sciences
- Current Assignee Address: JP Anagawa
- Agency: Husch Blackwell Sanders Welsh & Katz
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H2/04

Abstract:
A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
Public/Granted literature
- US20090311753A1 Method for amplifying genomic DNA Public/Granted day:2009-12-17
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