A method and system for filter calibration using fractional-N frequency synthesized signals are presented. The method may include generating an Local Oscillator (LO) signal by a Phase Locked Loop (PLL) circuit within a chip. A reference signal may be generated based on the generated LO signal and a synthesizer control signal. A frequency response for a filter circuit integrated within the chip may be calibrated by adjusting parameters associated with the filter circuit based on the generated LO signal. The system may include a single-chip multi-band RF receiver that enables generation of a LO signal by a PLL circuit within the single-chip, and enables calibration of a frequency response for a filter circuit integrated within the chip. A reference signal may be generated based on the generated LO signal and a synthesizer control signal. The frequency response may be calibrated by adjusting the filter based on the generated reference signal.