An add-in card fastener is provided. The fastener includes a base, a pressing part, a latch and a supporting part. The base is fixed on an inner surface of a computer case. The pressing part is pivotally connected to the base. The pressing part has a pin located on a first surface of the pressing part to press an add-in card on a motherboard. The supporting part is connected to the base. The latch is located on one side of the supporting part and presses a second surface of the pressing part when the pressing part presses the add-in card. The second surface of the pressing part is opposite the first surface of the pressing part.