Invention Grant
- Patent Title: Method of genome-wide nucleic acid fingerprinting of functional regions
- Patent Title (中): 功能区全基因组核酸指纹图谱
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Application No.: US11031765Application Date: 2005-01-07
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Publication No.: US07691614B2Publication Date: 2010-04-06
- Inventor: Periannan Senapathy
- Applicant: Periannan Senapathy
- Applicant Address: US WI Madison
- Assignee: Genome Technologies, LLC
- Current Assignee: Genome Technologies, LLC
- Current Assignee Address: US WI Madison
- Agency: DeWitt Ross & Stevens S.C.
- Agent Joseph T. Leone, Esq.
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68 ; C07H21/04 ; C07H21/00

Abstract:
A method of specifically amplifying desired regions of nucleic acid from a sample is provided. The method uses a plurality of first and second PCR primers, each having a region of fixed nucleotide sequence identical or complementary to a consensus sequence of interest and a region of randomized nucleotide sequence located 5′ to, 3′ to, anywhere within, or flanking the region of fixed nucleotide sequence; and then amplifying the nucleic acid present in the sample via PCR using the plurality of first and second PCR primers; whereby a subset of the first primers binds to the consensus sequence of interest wherever it occurs in the sample, and a subset of the second primers binds to the sample at locations removed from the first primers such that DNA regions flanked by the first primer and the second primer are specifically amplified.
Public/Granted literature
- US20050123980A1 Method of genome-wide nucleic acid fingerprinting of functional regions Public/Granted day:2005-06-09
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