A novel towelette dispensing assembly is presented comprising a housing and a frame wherein the housing is attached the frame, which in turn is releasably secured between the mounting brackets of a conventional toilet tissue roll holder. The frame includes parallel-spaced opposing support arms and mounting means. The mounting means is intended to be positioned on either side of a toilet tissue roll such that the expandable spindle provided as part of a conventional toilet tissue dispenser may engage or pass through said mounting means. The support arms of the frame assembly extend beyond the mounting means to bear against the wall or vertical surface to maintain the frame assembly in a substantially vertical position. One embodiment includes a novel tissue roll spindle. The towelette housing can take various shapes to accommodate a variety of conventional commercial towelette containers. The dispensing assembly is intended to be detachably coupled to a conventional toilet tissue holder in a manner that does not interfere with the dispensing of toilet tissue. By its coupling to the toilet tissue dispenser, the dispenser of this invention is conveniently located near the tissue roll and may be accessed without the user having to come in contact with the assembly.