Fish processing machines and methods for realigning fish during processing are disclosed herein. In one embodiment, a fish processing machine in accordance with the invention includes a fish processing unit for use with a processing machine having a fillet assembly and/or a skinner assembly. The processing unit includes an inspection assembly with a conveyor system operably coupled to the fillet assembly and/or skinner assembly and an alignment device operably coupled to the conveyor system. The conveyor system extends along a processing path to transport a fillet of fish that has been sliced from a trunk of the fish. The alignment device is positioned to automatically realign the fillet with respect to the processing path without manually manipulating the fillet before the fillet is transferred from the conveyor system to an inspection table.