Invention Grant
- Patent Title: AGPase promoter from rice and uses thereof
- Patent Title (中): 水稻AGPase启动子及其用途
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Application No.: US11762586Application Date: 2007-06-13
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Publication No.: US07700835B2Publication Date: 2010-04-20
- Inventor: Fumio Takaiwa , Leqing Qu
- Applicant: Fumio Takaiwa , Leqing Qu
- Applicant Address: JP Tsukuba-shi, Ibaraki
- Assignee: National Institute of Agrobiological Sciences
- Current Assignee: National Institute of Agrobiological Sciences
- Current Assignee Address: JP Tsukuba-shi, Ibaraki
- Agency: Knobbe, Martens, Olson & Bear, LLP
- Priority: JP2003-373815 20031031
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12N5/04 ; C12N15/82 ; A01H5/00

Abstract:
An objective of the present invention is to provide promoters having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. The present inventors isolated the promoter of ADP-Glucose pyrophosphorylase which is expressed in rice seeds, constructed binary vectors in which the ADP-Glucose pyrophosphorylase promoter is inserted upstream of the GUS reporter gene, and transformed rice using the Agrobacterium method. GUS expression level was used as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for the ADP-Glucose Pyrophosphorylase promoter. Expression was found in the embryo during early stage of maturation and in the entire seed during maturation. Expression in late stage of maturation in embryo was very high.
Public/Granted literature
- US20080313771A1 SEED-SPECIFIC GENE PROMOTERS AND USES THEREOF Public/Granted day:2008-12-18
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