Invention Grant
- Patent Title: Peroxide-driven cytochrome P450 oxygenase variants
- Patent Title (中): 过氧化物驱动的细胞色素P450加氧酶变体
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Application No.: US12287681Application Date: 2008-10-11
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Publication No.: US07704715B2Publication Date: 2010-04-27
- Inventor: Patrick C. Cirino , Frances H. Arnold
- Applicant: Patrick C. Cirino , Frances H. Arnold
- Applicant Address: US CA Pasadena
- Assignee: California Institute of Technology
- Current Assignee: California Institute of Technology
- Current Assignee Address: US CA Pasadena
- Agency: Gavrilovich Dodd & Lindsey LLP
- Agent Joseph R. Baker, Jr.
- Main IPC: C12N9/02
- IPC: C12N9/02 ; C12Q1/26 ; C12Q1/68 ; C12N15/00 ; C12N1/20 ; C12P21/04 ; C07H21/04
Abstract:
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.
Public/Granted literature
- US20090142821A1 Peroxide-driven cytochrome P450 oxygenase variants Public/Granted day:2009-06-04
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