Invention Grant
US07705137B2 Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same
失效
具有糜蛋白酶活性的微生物胰蛋白酶突变体和编码相同的核酸
- Patent Title: Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same
- Patent Title (中): 具有糜蛋白酶活性的微生物胰蛋白酶突变体和编码相同的核酸
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Application No.: US12022771Application Date: 2008-01-30
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Publication No.: US07705137B2Publication Date: 2010-04-27
- Inventor: Alan Klotz , Kimberly M. Brown , Elizabeth J. Zaretsky
- Applicant: Alan Klotz , Kimberly M. Brown , Elizabeth J. Zaretsky
- Applicant Address: US CA Davis
- Assignee: Novozymes, Inc.
- Current Assignee: Novozymes, Inc.
- Current Assignee Address: US CA Davis
- Agent Robert L. Starnes
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12N9/64
Abstract:
The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
Public/Granted literature
- US20090035820A1 Microbial Trypsin Mutants Having Chymotrypsin Activity And Nucleic Acids Encoding Same Public/Granted day:2009-02-05
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