A misfire determination device for a multi-cylinder internal combustion engine, of which an output shaft is connected, through a torsion element, to a downstream shaft downstream of the torsion element, includes: an first portion for detecting an output shaft rotational speed that is the rotational speed of the output shaft; a second portion for detecting a downstream shaft rotational speed that is the rotational speed of the downstream shaft; a third portion that calculates a component caused by an influence of resonance due to torsion of the torsion element on the output shaft rotational speed, based on the acquired output shaft rotational speed and the acquired downstream shaft rotational speed; and a fourth portion for determining the occurrence of the misfire in the internal combustion engine based on a rotational speed that is obtained by subtracting the calculated component from the detected output shaft rotational speed.