The present invention provides a file holder with a hanging mechanism, wherein the file holder includes a main body and a hanging mechanism. The hanging mechanism is arranged onto the inner wall of a front panel of the main body and includes a limiting portion, a hanging band and a locator. The first locating end of the locator can be meshed with or disengaged from the second locating end. The hanging band is penetrated into the limiting portion. The hanging band can be retractably concealed into the front panel, or pulled outwards in a hanging state. The hanging band can be limited by meshing of the first locating end with the second locating end or by abutting separately the first and second locating ends onto the first and second threading portions.