Invention Grant
- Patent Title: Methods of quantitative detection of genetic recombinants and standard molecules for the methods
- Patent Title (中): 方法定量检测遗传重组体和标准分子
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Application No.: US10423399Application Date: 2003-04-25
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Publication No.: US07709232B2Publication Date: 2010-05-04
- Inventor: Akihiro Hino , Takeshi Matsuoka , Hideo Kuribara , Tomoaki Yoshimura , Yoichiro Shindo , Satoshi Futo , Machiko Ogawa
- Applicant: Akihiro Hino , Takeshi Matsuoka , Hideo Kuribara , Tomoaki Yoshimura , Yoichiro Shindo , Satoshi Futo , Machiko Ogawa
- Applicant Address: JP Ibaraki JP Tokyo
- Assignee: National Food Research Institute,Asahi Breweries, Ltd.
- Current Assignee: National Food Research Institute,Asahi Breweries, Ltd.
- Current Assignee Address: JP Ibaraki JP Tokyo
- Agency: Hoffmann & Baron, LLP
- Priority: JP2000-326738 20001026
- Main IPC: C12P1/34
- IPC: C12P1/34 ; C12Q1/68 ; C07H21/02 ; C07H21/04 ; C07H21/00
Abstract:
The present invention relate to a method of detecting a genetic recombinant by using the PCR method. A method of quantitatively detecting method is provided whereby the total content ratio of the genetic recombinants and the individual content ratio of the genetic recombinant in a population containing plural genetic recombinant lines can be quantified. The method of the present invention comprises performing PCR for the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species corresponding to the recombinant using, as a standard molecule, a molecule containing the DNA sequence specific to the recombinant and the endogenous DNA sequence shared by the species on the single molecule, and determining the content ratio of the number of molecules thereof.
Public/Granted literature
- US20040005605A1 Methods of quantitative detection of genetic recombinants and standard molecules for the methods Public/Granted day:2004-01-08
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