Invention Grant
US07709624B2 Process for producing recombinant protein in bacterium belonging to the genus Rhodococcus
有权
用于在属于红球菌属的细菌中产生重组蛋白的方法
- Patent Title: Process for producing recombinant protein in bacterium belonging to the genus Rhodococcus
- Patent Title (中): 用于在属于红球菌属的细菌中产生重组蛋白的方法
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Application No.: US10553979Application Date: 2004-04-19
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Publication No.: US07709624B2Publication Date: 2010-05-04
- Inventor: Nobutaka Nakashima , Tomohiro Tamura
- Applicant: Nobutaka Nakashima , Tomohiro Tamura
- Applicant Address: JP Tokyo
- Assignee: National Institute of Advanced Industrial Science and Technology
- Current Assignee: National Institute of Advanced Industrial Science and Technology
- Current Assignee Address: JP Tokyo
- Agency: Foley & Lardner LLP
- Agent Stephen A. Bent
- Priority: JP2003-116280 20030421
- International Application: PCT/JP2004/005585 WO 20040419
- International Announcement: WO2004/094633 WO 20041104
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12N15/63 ; C12N1/21

Abstract:
Provided is an expression vector capable of constitutive expression of a foreign gene in a bacterium belonging to the genus Rhodococcus. The vector is a constitutive expression vector for a bacterium belonging to the genus Rhodococcus comprising: DNA comprising a nucleotide sequence of a mutated TipA gene promoter where a mutation is incorporated into a −10 region sequence of a TipA gene promoter, the mutated TipA gene promoter being capable of thiostrepton-independent and constitutive expression of a gene located downstream thereof; a promoter sequence for the constitutive expression of a foreign gene; a ribosome-binding site sequence located downstream of the promoter sequence; and a multiple-cloning site sequence capable of incorporating a foreign gene therein, located downstream of the ribosome-binding site sequence.
Public/Granted literature
- US20070004004A1 Process for producing recombinant protein in bacterium belonging to the genus rhodococcus Public/Granted day:2007-01-04
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