A single-ended operational amplifier includes an output stage, a first transconductance amplifier and a second transconductance amplifier. In an offset cancellation mode, two inputs of the first transconductance amplifier are supplied with a reference voltage to sink two currents from two inputs of the output stage respectively, the output stage generates a third current according to the difference between the two currents to charge a capacitor, and the second transconductance amplifier generates two currents according to the voltage in the capacitor to make currents in the two inputs of the output stage equal to each other, thereby canceling the offset of the single-ended operational amplifier.