Invention Grant
US07718403B2 Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
有权
通过DNA聚合过程产生的全基因组和整个转录组文库的扩增和分析
- Patent Title: Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
- Patent Title (中): 通过DNA聚合过程产生的全基因组和整个转录组文库的扩增和分析
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Application No.: US10795667Application Date: 2004-03-08
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Publication No.: US07718403B2Publication Date: 2010-05-18
- Inventor: Emmanuel Kamberov , Tong Sun , Eric Bruening , Jonathon H. Pinter , Irina Sleptsova , Takao Kurihara , Vladimir L. Makarov
- Applicant: Emmanuel Kamberov , Tong Sun , Eric Bruening , Jonathon H. Pinter , Irina Sleptsova , Takao Kurihara , Vladimir L. Makarov
- Applicant Address: US MI Ann Arbor
- Assignee: Rubicon Genomics, Inc.
- Current Assignee: Rubicon Genomics, Inc.
- Current Assignee Address: US MI Ann Arbor
- Agency: Fulbright & Jaworski L.L.P.
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The present invention regards a variety of methods and compositions for whole genome amplification and whole transcriptome amplification. In a particular aspect of the present invention, there is a method of amplifying a genome comprising a library generation step followed by a library amplification step. In specific embodiments, the library generating step utilizes specific primer mixtures and a DNA polymerase, wherein the specific primer mixtures are designed to eliminate ability to self-hybridize and/or hybridize to other primers within a mixture but efficiently and frequently prime nucleic acid templates.
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